KMID : 0363620220430020001
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Journal of Korean Oriental Medicine 2022 Volume.43 No. 2 p.1 ~ p.7
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CD206+ dendritic cells might be associated with Heat-pattern and induced regulatory T cells after treatment with bee venom
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Jung Woo-Sang
Kwon Seung-Won Yang Jung-Yun Jin Chul Cho Seung-Yeon Park Seong-Uk Moon Sang-Kwan Park Jung-Mi Ko Chang-Nam Bae Hyun-Su Cho Ki-Ho
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Abstract
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Objectives: Bee venom (BV) is a widely used therapy in Traditional East Asian Medicine (TEAM). We previously reported that BV was clinically effective for treating Parkinson¡¯s disease, that phospholipase A2 (PLA2) was the main component of BV, and that it induced regulatory T cells (Tregs) by binding CD206 on dendritic cells (DCs). Therefore, we aimed to reconfirm our findings in human blood samples and investigate the relationship between CD206+ DCs and clinical syndrome differentiation in TEAM.
Methods: We surveyed 100 subjects with questionnaires on cold-heat patternization and obtained their blood samples. The obtained human peripheral blood monocytes (hPBMCs) were washed with phosphate-buffered saline (PBS). After resuspension with ex vivo media, numbers of cells were counted. Tregs were counted after culturing the samples in a 37¡É CO2 incubator for 72 h.
Results: We divided the subjects into a relatively high CD206+ group or a relatively low CD206+ group. The heat factor scores of high CD206+ group were significantly higher than that of low CD206+ group (high vs low: 239.2 ¡¾ 54.1 vs 208.4 ¡¾ 55.1, p=0.023). After culturing with PLA2, Tregs increased in the high CD206+ group but decreased in the low CD206+ group.
Conclusion: In this study, we reconfirm that CD206+ DCs induced Treg differentiation by incubating human blood samples with PLA2 and that they showed an association with syndrome differentiation, especially with heat patterns, in TEAM. A heat pattern in TEAM might be one indication for PLA2 therapy because its score was elevated in the high CD206+ group.
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KEYWORD
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Bee venom, CD206, Pattern Identification, phospholipase A2, Regulatory T cell
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